Molecular Characterization of Moraxella Catarrhalis and it's Antibiotics Susceptibility Patterns from Different Respiratory Tract Infection Patient's Clinical Samples

IntroductionMoraxella catarrhalis is a gram negative, oxidase positive, cocci mainly causing upper and lowerrespiratory tract infections. The RND family efflux pumps lead to multidrug resistance in most ofthe negative bacteria. One of the well- known M. arcAB oprMsystem. objective this study was investigate antibiotic andto determine its dependence on pump. MethodsIn study, 283 different respiratory infection samples processed out that 73 were confirmedby biotyping molecular characterization as catarrhalis. susceptibility testswere performed by disc diffusion method according CLSI. Molecular formultidrug isolates also done along with genes responsible for pumps. PCRwas acrAB oprM genes. factor association pump withantibiotic investigated using phenylalanine argine β-naphthylamide. ResultsThe antibiotics result showed 12 MDR, selectively takenfor PCR 16SrRNA specific primer. MDR further confirmed PCR. Theamplification results acra, acrb oprm having resistancegenes band size products seen 2% agrose gel. highest towards drugsviz penicillin, ampicillin, amoxicillin, amoxiclave, cotrimoxazole, cefazolin, cefuroxime, ceftrixoneand cefepime ConclusionsThe Moraxella one major causes infection.The drug species increasing day day. 90% have andoprM multiple resistance. inhibitorhas important clinical significance proper treatment.